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Sequencing by Ligation Variation with Endonuclease V, Deoxyinosine, and SAWTooth The Sequencing Analysis Workbench Tool

SBL data analysis

SBL data analysis

Following the cyclic SBL, an analysis is performed of how many times each tag mapped to the genome. One of the more significant benefits gained by increasing tag length from 13 to 20 bases is that far fewer tags must be discarded because they do not map uniquely. At a tag length of 13 bases, only 57.2% of the tags are used, compared to 85.6% at a tag length of 20, thus effectively increasing throughput.

Credits: Antoine Ho – University of New Mexico