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Novel Silica Nanoparticles Enhance Gene Delivery to the Spinal Cord: Potential to Control Pathological Pain
Protocells are Not Toxic to Raw 264.7 Macrophage Cells
24 well plates seeded with 1 × 10 5 cells were treated for 24 hours with protocells lacking a lipid bilayer (A, B) or a lipid bilayer composed of DOTAP: CHOL 1:1 (C, D) DOPC (E, F) or DOTAP (G, H). Cells were then subjected to a Life/Dead cell viability assay (Invitrogen, Inc.). Live cells, stained green (Calcein-AM) (A, C, E, G), made up 95% of the total population, while dead cells, stained red (Ethidium homodimer) (B, D, F, H), composed only 5%. Confocal imaging at 10X.
Credits: Ellen Dengler, University of New Mexico
